In denitrification the reduction of nitrate to nitrite is catalyzed by the membrane bound complex nitrate reductase NarGHI (González et al., 2006, Moreno-Vivian et al., 1999). In several non-denitrifying bacteria membrane bound Nar can also function as respiratory nitrate reductase ( Richardson et al., 2001 , Zumft, 1997 ).
2. Zinc to the remaining tubes that did not turn red. Nitrate Reduction: after adding Nitrate A & B. Positive Results & bacteria. Negative Results & bacteria. Nitrate A & B: Positive: E. coli (red) [NO3-->NO2] Negative (Questionable): P. aeruginosa & A. faecalis (yellow) Nitrate Reduction: after adding Zinc.I. Oxidative-fermentative test using OF media with glucose A. Inoculation of media Two tubes of oxidative-fermentative medium are inoculated by stabbing “half way to the bottom” (8) or ¼-inch from the bottom (6) with the test organism. Overlay one of the two tubes with 1 cm of mineral oil (8) (Fig. 1, 2, 3). . 569 735 935 899 126 80 63 27